ABSTRACT

In recent years, the development of therapeutic monoclonal antibodies (mAbs) has gained considerable importance, and these molecules have become increasingly important in the worldwide biotechnological drug market. Many biotechnology companies are investing in monoclonal antibody production for treatment of various diseases. Bioreactor production and purification are the two major steps in the manufacturing of these antibodies. Current purification methods used for these molecules are extensively discussed in this review. The basic recovery and purification processes for monoclonal antibodies such as cell harvesting, Protein A affinity chromatography, and additional polishing steps are all summarized.